Nanogram-scale derivatization of hydroxy groups for highly sensitive HPLC/MS/CD detection

Abstract
A strategy for performing submicrogram-scale structural studies of saponins and related compounds is worked out by: (i) naphthoylation to sensitize HPLC detection by fluorescence as well as configurational studies by exciton coupled CD; and (ii) ω-cyanoundecanoylation to increase LC/MS sensitivity (ca. 100-fold).

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