Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells.
Open Access
- 1 February 1988
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 8 (2) , 764-769
- https://doi.org/10.1128/mcb.8.2.764
Abstract
We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and overexpressed 1,000-fold. This ODC vector was a dominant marker in a variety of cell types and displayed at least 300-fold amplification in wild-type Chinese hamster ovary cells.This publication has 35 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- POLYAMINESAnnual Review of Biochemistry, 1984
- Identification of two distinct regulatory regions adjacent to the human β-interferon geneCell, 1983
- Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA geneJournal of Molecular Biology, 1982
- Gene amplification and gene correction in somatic cellsCell, 1982
- Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmidsNature, 1981
- Transforming DNA integrates into the host chromosomeCell, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973
- A membrane-filter technique for the detection of complementary DNABiochemical and Biophysical Research Communications, 1966