Characterization of two isolates of a tobamovirus fromLychnis albarelated to Holmes' ribgrass strain

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Abstract
Two isolates of a tobamovirus from Lychnis alba collected in the Okanagan Valley in British Columbia Canada, were described. These isolates (L1 and L2) were close related to tobacco mosaic virus Holmes'' ribgrass (HR) isolate on the basis of their amino acid composition. The two isolates differed by their symptomatology on Samsun tobacco and by their sensitivity to in vitro decapsidation conditions. Analysis of viral RNAs with fingerprint techniques showed a complexity similar to other well-known strains of TMV and no difference between the two isolates. The coat protein of both isolates behaved like a 16000 polypeptide as characterized by gradient polyacrylamide gel electrophoresis. Several immunological cross-reactions were obtained with different TMV isolates. This is the second report of HR-related tobamovirus isolates from wild Lychnis alba (Caryophyllaceae).