Mechanism of tRNA-synthetase recognition: role of terminal A
Open Access
- 1 January 1977
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 4 (7) , 2253-2262
- https://doi.org/10.1093/nar/4.7.2253
Abstract
The function of the terminal A of tRNAPhe(yeast) with respect to complex formation with the cognate aminoacyl-tRNA synthetase has been studied using equilibrium and fast kinetic techniques. Removal of the terminal A influences the equilibrium parameters of the tRNA-synthetase interaction only slightly, the mechanism of complex formation, however, is changed significantly. The binding mechanism of unmodified tRNAPhe comprises a recombination step and a consecutive conformational change. In contrast, the reaction between and the cognate synthetase is characterized by a simple one step mechanism. It is concluded that the terminal A is responsible for the ocurrence of the conformational change of the tRNA-synthetase complex. The conformational change is interpreted as a proper alignment of the terminal A of the tRNA to the active site of the synthetase.Keywords
This publication has 16 references indexed in Scilit:
- Hydrolytic action of aminoacyl-tRNA synthetases from Baker's yeast: "chemical proofreading" preventing acylation of tRNAIle with misactivated valineBiochemistry, 1976
- Mechanism of Discrimination between Cognate and Non-cognate tRNs by Phenylalanyl-tRNA:Synthetase from YeastEuropean Journal of Biochemistry, 1976
- Distinct Steps in the Specific Binding of tRNA to Aminoacyl-tRNA Synthetase. Temperature-Jump Studies on the Serine-Specific System from Yeast and the Tyrosine-Specific System from Escherichia coliEuropean Journal of Biochemistry, 1976
- Enzyme hyperspecificity. Rejection of threonine by the valyl-tRNA synthetase by misacylation and hydrolytic editingBiochemistry, 1976
- Antico-operative binding of bacterial and mammalian initiator tRNAMet to methionyl-tRNA synthetase from Escherichia coliJournal of Molecular Biology, 1976
- On the Interaction of Seryl-tRNA Synthetase with tRNASer. A Contribution to the Problem of Synthetase-tRNA RecognitionEuropean Journal of Biochemistry, 1976
- Equivalent and Non‐equivalent Binding Sites for tRNA on Aminoacyl‐tRNA SynthetasesEuropean Journal of Biochemistry, 1975
- Biological Activity of Escherichia coli tRNAPhe Modified in Its C‐C‐A TerminusEuropean Journal of Biochemistry, 1972
- Isolation and Properties of tRNA Nucleotidyl Transferase from YeastEuropean Journal of Biochemistry, 1971
- A simple procedure for the purification of yeast phenylalanine transfer RNABiochemical and Biophysical Research Communications, 1968