The calmodulin-stimulated ATPase of maize coleoptiles is a 140000-Mr polypeptide
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 176 (2) , 283-285
- https://doi.org/10.1007/bf00392457
Abstract
The calmodulin-stimulated ATPase of maize (Zea mays L.) coleoptiles has been purified by calcium-dependent binding to a calmodulin affinity column. In the presence of protease inhibitors (phenylmethylsulfonylfluoride and chymostatin) a polypeptide of relative molecular mass (Mr) 140000 (±10000) is obtained on sodium-dodecylsulphate polyacrylamide gels. This polypeptide is recognised specifically by an affinity-purified polyclonal antibody to mammalian calmodulin-stimulated calcium-pumping ATPases and is of similar Mr to the erythrocyte-membrane calcium pump (138000 Mr).Keywords
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