Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity
Top Cited Papers
- 9 March 2001
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 291 (5510) , 1965-1969
- https://doi.org/10.1126/science.1057269
Abstract
To study the mechanisms underlying the high pathogenicity of Ebola virus, we have established a system that allows the recovery of infectious virus from cloned cDNA and thus permits genetic manipulation. We created a mutant in which the editing site of the gene encoding envelope glycoprotein (GP) was eliminated. This mutant no longer expressed the nonstructural glycoprotein sGP. Synthesis of GP increased, but most of it accumulated in the endoplasmic reticulum as immature precursor. The mutant was significantly more cytotoxic than wild-type virus, indicating that cytotoxicity caused by GP is down-regulated by the virus through transcriptional RNA editing and expression of sGP.Keywords
This publication has 25 references indexed in Scilit:
- Molecular Characterization of Guinea Pig-Adapted Variants of Ebola VirusVirology, 2000
- Differential induction of cellular detachment by envelope glycoproteins of Marburg and Ebola (Zaire) virusesJournal of General Virology, 2000
- Identification of the Ebola virus glycoprotein as the main viral determinant of vascular cell cytotoxicity and injury.Nature Medicine, 2000
- Characterization of the L gene and 5' trailer region of Ebola virus.Journal of General Virology, 1999
- The virion glycoproteins of Ebola viruses are encoded in two reading frames and are expressed through transcriptional editing.Proceedings of the National Academy of Sciences, 1996
- GP mRNA of Ebola Virus Is Edited by the Ebola Virus Polymerase and by T7 and Vaccinia Virus Polymerases1Virology, 1995
- The GP‐protein of Marburg virus contains the region similar to the ‘immunosuppressive domain’ of oncogenic retrovirus P15E proteinsFEBS Letters, 1993
- The envelope glycoprotein of Ebola virus contains an immunosuppressive‐like domain similar to oncogenic retrovirusesFEBS Letters, 1992
- Infectious defective interfering particles of VSV from transcripts of a cDNA cloneCell, 1992
- A pseudoknot-like structure required for efficient self-cleavage of hepatitis delta virus RNANature, 1991