STUDIES ON A SIMPLE LIMULUS TEST, A SLIDE METHOD

  • 1 January 1977
    • journal article
    • research article
    • Vol. 47  (6) , 523-524
Abstract
A simple micromethod of Limulus test [for bacterial endotoxin determination] was studied. A 20 .mu.l aliquot of Pregel lysate solution and the same amount of sample are dropped on the same place on the silicone coated slide glass and mixed well. Then it is incubated at 37.degree. C for 30 min in a moisture chamber. After the incubation, the vibration of the droplet surface is observed. When the vibration is no longer observed, it is judged as positive reaction, and when the vibration is still observed as before the incubation, it is judged as negative reaction. Compared with the conventional tube method, this method has certain advantages: the judgement of the result is relatively objective, shorter incubation period is enough for the reaction to take place and smaller amount of Limulus lysate is enough for each sample. Besides these merits, the detection limit of this method is not inferior to that of the tube method.

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