Reverse dot blot probes for the screening of β-thalassernia mutationsin Asians and American blacks

1 January 1994

journal article
research article
Published by Hindawi Limited in Human Mutation

Vol. 3 (1) , 59-63
https://doi.org/10.1002/humu.1380030110

Abstract

We have optimized a battery of reverse dot blot oligonucleotide probes for detecting the most common β-globin gene mutations in Asians and American Blacks. These probes allow a high degree of coverage of mutant chromosomes in these two populations and are useful in mutation screening and prenatal diagnosis. When coupled with the Mediterranean subset of probes, a 95% worldwide coverage of β-thalassemia mutations should be possible. The use of these probes in the reverse dot blot system should allow the distribution of premade strips and application to automated screening.

Keywords

This publication has 5 references indexed in Scilit:

Rapid and simultaneous typing of hemoglobin S, hemoglobin C, and seven Mediterranean beta-thalassemia mutations by covalent reverse dot-blot analysis: application to prenatal diagnosis in Sicily
Blood, 1993
The spectrum of cystic fibrosis mutations
Trends in Genetics, 1992
Detection of multiple cystic fibrosis mutations by reverse dot blot hybridization: a technology for carrier screening
Human Genetics, 1992
Gene Defects in β‐Thalassemia and Their Prenatal Diagnosis
Annals of the New York Academy of Sciences, 1990
Genetic analysis of amplified DNA with immobilized sequence-specific oligonucleotide probes.
Proceedings of the National Academy of Sciences, 1989