Identification of an operon involved in the assimilatory nitrate‐reducing system of Azotobacter vineiandii
- 1 June 1993
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 8 (6) , 1145-1153
- https://doi.org/10.1111/j.1365-2958.1993.tb01659.x
Abstract
A number of mutants lacking nitrate reductase (Nas-) or nitrite reductase (Nis-) activities have been isolated and characterized. An operon including two new genes (nasA and nasB) has been defined and cloned from an Azotobacter vinelandii gene bank. nasA encodes for nitrite reductase apoenzyme, whereas nasB is specific for nitrate reductase activity. Nitrate reductase exerts a regulatory effect on nasAB.Keywords
This publication has 32 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Nucleotide sequence, organisation and structural analysis of the products of genes in the nirB–cysG region of the Escherichia coli K‐12 chromosomeEuropean Journal of Biochemistry, 1990
- New derivatives of transposon tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in Gram-negative bacteriaGene, 1989
- The Enzymes Associated with DenitrificationAnnual Review of Microbiology, 1988
- Mutants of Azotobacter vinelandii altered in the regulation of nitrate assimilationArchiv für Mikrobiologie, 1987
- The Assimilatory Nitrate-Reducing System and its RegulationAnnual Review of Plant Physiology, 1981
- Isolation and analysis of mutants of Pseudomonas aeruginosa unable to assimilate nitrateArchiv für Mikrobiologie, 1979
- Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in transProceedings of the National Academy of Sciences, 1979
- A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samplesAnalytical Biochemistry, 1978
- Reduced nicotinamide–adenine dinucleotide–nitrite reductase from Azotobacter chroococcumBiochemical Journal, 1973