MULTIPLE HEMATOPOIETIC LINEAGES ARE FOUND AFTER STIMULATION OF MOUSE BONE-MARROW PRECURSOR CELLS WITH INTERLEUKIN-3

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 117  (2) , 171-179
Abstract
When the murine T-lymphocyte clone L2 is stimulated with concanavalin A, it secretes at least 2 distinct factors that affect hemopoietic precursor cells, interleukin 3 (IL3) and granulocyte/macrophage colony-stimulating factor (GM-CSF). IL3 accounts for approximately 10% of the colony-stimulating activity in L2-cell-conditioned medium. The IL3 secreted by L2 cells is similar antigenically to the IL3 secreted by WEHI-3 cells. Like the IL3 from WEHI-3 cells, IL3 secreted by L2 cells does not bind to DEAE Sephacel and can be separated from the L2-cell GM-CSF, which does bind to DEAE. By assessment of the functional, morphologic, surface phenotypic and cytochemical characteristics of bone marrow cells 6 days after stimulation with IL3 in liquid culture, 4 hemopoietic lineages were found, including macrophage, neutrophilic granulocyte, megakaryocyte and basophil/mast cell. When bone marrow cells were stimulated with IL3 in semisolid medium, several types of colonies were found, including mixed colonies containing macrophage, megakaryocyte, and granulocyte lineages.