A Direct and efficient synthesis method for dumbell-shaped linear DNA using PCR in vitro
Open Access
- 1 September 2003
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Symposium Series
- Vol. 3 (1) , 191-192
- https://doi.org/10.1093/nass/3.1.191
Abstract
A linear, covalently-closed, dumbbell-shaped DNA vector including a transcription unit is known to have both biological stability and safety and is expected to be useful for gene therapy. We established an easy, quick, and large preparative synthetic method of modified- and unmodified-dumbbell DNA using an intramolecular cyclization at the DNA termini.Keywords
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