Selective plane illumination microscopy techniques in developmental biology
Top Cited Papers
Open Access
- 15 June 2009
- journal article
- review article
- Published by The Company of Biologists in Development
- Vol. 136 (12) , 1963-1975
- https://doi.org/10.1242/dev.022426
Abstract
Selective plane illumination microscopy (SPIM) and other fluorescence microscopy techniques in which a focused sheet of light serves to illuminate the sample have become increasingly popular in developmental studies. Fluorescence light-sheet microscopy bridges the gap in image quality between fluorescence stereomicroscopy and high-resolution imaging of fixed tissue sections. In addition, high depth penetration, low bleaching and high acquisition speeds make light-sheet microscopy ideally suited for extended time-lapse experiments in live embryos. This review compares the benefits and challenges of light-sheet microscopy with established fluorescence microscopy techniques such as confocal microscopy and discusses the different implementations and applications of this easily adaptable technology.Keywords
This publication has 42 references indexed in Scilit:
- Reconstruction of Zebrafish Early Embryonic Development by Scanned Light Sheet MicroscopyScience, 2008
- High-speed imaging of developing heart valves reveals interplay of morphogenesis and functionDevelopment, 2008
- Highly inclined thin illumination enables clear single-molecule imaging in cellsNature Methods, 2008
- Zebrafish model for human long QT syndromeProceedings of the National Academy of Sciences, 2007
- Multi-view image fusion improves resolution in three-dimensional microscopyOptics Express, 2007
- Design and quantitative resolution measurements of an optical virtual sectioning three-dimensional imaging technique for biomedical specimens, featuring two-micrometer slicing resolutionJournal of Biomedical Optics, 2007
- High-resolution episcopic microscopy: a rapid technique for high detailed 3D analysis of gene activity in the context of tissue architecture and morphologyBrain Structure and Function, 2006
- Serial Block-Face Scanning Electron Microscopy to Reconstruct Three-Dimensional Tissue NanostructurePLoS Biology, 2004
- Three-Dimensional Imaging by Deconvolution MicroscopyMethods, 1999
- Orthogonal‐plane fluorescence optical sectioning: Three‐dimensional imaging of macroscopic biological specimensJournal of Microscopy, 1993