Enteric bacterial antigens activate CD4+ T cells fromscid mice with inflammatory bowel disease
- 1 January 2001
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 31 (1) , 23-31
- https://doi.org/10.1002/1521-4141(200101)31:1<23::aid-immu23>3.0.co;2-2
Abstract
Scid mice transplanted with CD4+ T cells from congenic donor mice develop a chronic and lethal inflammatory bowel disease (IBD) 2–3 months post-transplantation. In the present study we have investigated the response of CD4+ T cells from scid mice with colitis against fecal extracts. Our results show that in contrast to CD4+ T cells from normal BALB/c mice, CD4+ T cells from scid mice with colitis proliferate strongly in response to antigen-presenting cells (APC) pulsed with fecal extracts. The IBD-associated T cells did not respond to either extracts from food antigens or fecal extracts from germ-free mice, which indicates that they recognize bacterial antigens in the fecal extracts. CD4+ T cells isolated from the colonic lamina propria of scid mice 3 weeks post transplantation also responded vigorously to fecal extracts, demonstrating that reactive CD4+ T cells are present in the gut mucosa of transplanted scid mice prior to clinical manifestations of IBD. CD4+ T cells activated by fecal extracts produced high amounts of IL-2 and IFN-γ, intermediate amounts of IL-4 and low amounts of IL-10, consistent with a Th1 profile. The proliferative reactivity towards fecal extracts was restricted by MHC class II molecules and dependent on antigen processing, as the response could be blocked by anti-MHC class II antibodies or a short fixation of the APC. This study demonstrates that class II-restricted CD4+ Th1 cells, which recognize enteric bacterial antigens, infiltrate the gut mucosa and spleen of transplanted scid mice prior to and during the course of colitis.Keywords
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