An Enzyme-Immobilization Procedure for the Analysis of Enzyme-Inhibiting Chemicals in Water

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Abstract
Cholinesterase and urease were mixed individually with gelatin and immobilized onto the inside surface of glass capillary tubes. After the gelatin-enzyme mixture dried, water samples containing various enzyme inhibiting test chemicals were pumped through tubes to enable interaction to proceed between foreign agents and enzymes. Substrate for each enzyme was later passed through tubes and any difference in relative enzyme activity was determined. This procedure was useful for health and environmental studies, particularly in rapid testing for micromolar and nanomolar quantities of enzyme-inhibiting pollutants in natural waters and complex effluents.