Growth Responses of a Sulfonamide-requiring Mutant Strain of Neurospora

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Abstract
A mutant strain of N. crassa is reported in which the antagonistic roles of p-aminobenzoic acid and the sulfonamides have been reversed to the extent that sulfonamides are essential to growth at 34[degree] C or higher (opt. conc. about 2 X 10-4 M sulfanilamide), and p-aminobenzoic acid inhibits growth at concs. as low as 10-6 M. A double mutant, combining this gene with Tatum and Beadle''s aminobenzoicless gene, requires both sulfonamides and p-aminobenzoic acid in a molar ratio of about 1,000:1, an excess of either analog inhibiting growth in a competitive manner. The possibility that sul-fanilamide is utilized as a metabolite is discussed.
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