Iron-chelating Hydroxamic Acid (Schizokinen) Active in Initiation of Cell Division in Bacillus megaterium

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Abstract
Bacillus megaterium ATCC 19213 secretes a cell division-initiating "schizokinen " (SK) which accumulates during its culture cycle to a concentration inversely proportional to the iron added to a sucrose -mineral salts medium. Secreted SK was purified from culture filtrates as a red Fe (III) chelate, and a fraction with similar biological properties was obtained from whole cells. Infrared spectra of SK, and analyses of unhydrolyzed and acidhydrolyzed preparations indicated it to be a secondary hydroxamate; visible absorption maxima of the ferric complex showed pH dependency typical of ferric monohydroxamates. Schizokinen preparations from cultures grown at" normal " and a low Fe concentrations were similar biologically and in certain of their chemical properties, but their RF values and infrared spectra suggested nonidentity. Significant lag reduction of B. megaterium was effected by 0.2 m,[mu] of SK per ml; the Fe (III)-SK chelate and" iron-free " SK were equally effective. A 50-m[mu] amount produced half-maximal growth response of the siderochrome auxotroph, Arthrobacter JG-9. Schizokinen also overcame ferrimycin-A inhibition of three Bacillus species. These properties relate the B. megaterium schizokinen to the trihydroxamate siderochromes, although SK appears to be a monohydroxamate.