The Use of Various Substrates for Large-Scale Production of Fusarium oxysporum f. sp. cannabis Inoculum

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Abstract
Chlamydospore formation was enhanced in F. oxysporum f. sp. cannabis [for biological context of Cannabis sativa] in an aqueous solution individually amended with beef extract, soytone, proteose peptone #3, glycine, .beta.-alanine, succinate, lactate, or NaNO3. Chlamydospore formation was less with glucose (NH4)2SO4, tyrosine and tryptone. The ideal defined medium for chlamydospore formation was a solution of glycine-succinate-NaNO3. Diffusates from alfalfa straw, cottonseed meal and soybean oil meal induced chlamydospore formation, but few spores were formed with diffusates from sugar beet pulp, barley straw or safflower meal. Large-scale inoculum production by the fungus was achieved by culturing the fungus on a mixture of barley straw plus either the glycine-succinate-NaNO3 solution, alfalfa straw, cottonseed meal or soybean meal. Chlamydospores produced on the glycine-succinate-NaNO3-barley straw substrate retained their disease potential over a 6 mo. period at room temperature.

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