Abstract
The changes in pulmonary blood volume/unit wt. of lung were detd. in guinea pigs at intervals from 5 min. to 7 hrs. after birth. This was done by labeling the blood of the fetuses with radioiron and then comparing the radioactivity of lung tissue between breathing and nonbreathing litter-mates. The labeling was accomplished by the intraperit. admn. of ferrous sulfate to the pregnant mother 10-14 days before term. Rapid freezing of the newborn made possible the sampling of lung tissue without lung collapse or blood loss. After 5 min. of breathing the increase in pulmonary blood volume was approx. 24% and after 7 hrs. the increase avg. 85%.

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