Ribostamycin production by a mutant of butirosin producing bacteria.

Abstract
By the use of an improved colony selection technique, xylostasin and ribostamycin producing mutants were isolated from nitrosoguanidine treated Bacillus circulans B15M, a producer of butirosins A and B. Among these structurally related aminoglycosides, ribostamycin is the well-known product of a Streptomyces and was not isolated as a bacterial metabolite. A selected mutant of strain 306, which produces xylostasin and ribostamycin, was further mutagenized in expectation of getting an improved strain having the ability to accumulate a large amount of ribostamycin in the culture broth. One mutant, strain 451, derived from strain 306, produced ribostamycin free of xylostasin.

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