MICA exon 5 microsatellite typing by DNA heteroduplex analysis: a new polymorphism in the transmembrane region
- 1 March 1998
- journal article
- Published by Wiley in Tissue Antigens
- Vol. 51 (3) , 309-311
- https://doi.org/10.1111/j.1399-0039.1998.tb03108.x
Abstract
MICA (MHC class I chain‐related gene A) is localized 47 kb upstream from HLA‐B on the short arm of chromosome 6. It has been postulated that MICA protein folds similarly to the class I chain and may have the capacity to bind short ligands. Short tandem repeats (STR) within the transmembrane (TM) region of this gene have been described and five alleles consisting of 4 to 9 GCT codons, each encoding an alanine residue have been defined. We have applied DNA heteroduplex analysis to type MICA trinucleotide repeats in order to develop a simple and reliable method for their identification. This approach allowed the characterization of all MICA alleles. Moreover, a new polymorphism within the TM region was identified.Keywords
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