Characterization of a major late herpes simplex virus type 1 mRNA

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Abstract
A major, late 6 kilobase (kb) mRNA mapping in the large unique region of herpes simplex virus type 1 (HSV-1) was characterized by using 2 recombinant DNA clones, one containing EcoRI fragment G (0.190-0.30 map U) in .lambda.. WES.B and one containing HindIIIfragment J (0.181-0.259 map U) in pBR322. This 6 kb mRNA had its 3'' end to the left of 0.231 on the prototypical arrangement of the HSV-1 genome and was transcribed from right to left. It was bounded on both sides by regions containing a large number of distinct mRNA species and its 3'' end was partially colinear with a 1.5 kb mRNA which encoded a 35,000 dalton polypeptide. The 6 kb mRNA encoded a 155,000 dalton polypeptide which was shown to be the only one of this size detectable by hybrid-arrested translation encoded by late polyadenylated polyribosomal RNA. The S1 nuclease mapping experiments indicated that there were no introns in the coding sequence for this mRNA and that its 3'' end mapped .apprx. 800 nucleotides to the left of the BglII site at 0.231; its 5'' end extended very close to the BamHI site at 0.266.