Electric microassays of glucose, uric acid and cholesterol using peroxidase adsorbed on a carbon electrode.

Publisher Website
Google Scholar
Abstract
Highly sensitive electrochemical methods for the assays of glucose, uric acid and total cholesterol in serum were established by using peroxidase adsorbed on a carbon electrode. Glucose, uric acid and cholesterol were oxidized by glucose oxidase, uricase and cholesterol oxidase, respectively, to give a hydrogen peroxide, which was measured by means of a hydrogen peroxide sensor using peroxidase adsorbed on a carbon electrode. Each method required 5 .mu.l of serum, and the detection limits of glucose, uric acid and total cholesterol were 0.10 .mu.g (0.55 nmol), 0.05 .mu.g (0.295 nmol), and 0.25 .mu.g (0.650 nmol) per assay tube, respectively.