The ribosomal spacer inXenopus laevisis transcribed as part of the primary ribosomal RNA
- 1 January 1986
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 14 (15) , 6041-6052
- https://doi.org/10.1093/nar/14.15.6041
Abstract
S1 mapping of Xenopus laevis ribosomal RNA transcripts, both in oocyte microinjection experiments and in vivo, shows that all but 212 bp of the so-called "non-transcribed" spacer (NTS) of the ribosomal DNA repeat is transcribed as part of the primary ribosomal transcript. The 40S pre-ribosomal RNA (pre-rRNA) is therefore a processing intermediate. The primary ribosomal transcript co-terminates with the previously described spacer transcripts [Moss], at a site 213 bp upstream of the 40S pre-rRNA initiation site. This mode of transcription suggests a simple mechanism for the recently proposed phenomenon of "readthrough-enhancement", [Moss et al, Moss], where readthrough transcription from an upstream gene may enhance transcription of a gene immediately downstream in the tandem ribosomal repeat.Keywords
This publication has 15 references indexed in Scilit:
- Characterization of three sites of RNA 3$prime; end formation in the Xenopus ribosomal gene spacerCell, 1986
- Transcription of mouse rDNA terminates downstream of the 3′ end of 28S RNA and involves interaction of factors with repeated sequences in the 3′ spacerCell, 1985
- The promotion of ribosomal transcription in eukaryotes.1985
- Spacer sequences downstream of the 28S RNA coding region are part of the mouse rDNA transcription unitNucleic Acids Research, 1985
- Chromatin structure of Xenopus rDNA transcription termination sitesChromosoma, 1982
- Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoterCell, 1982
- Transcription of cloned Xenopus ribosomal genes visualised after injection into oocyte nucleiNature, 1978
- Sites of transcription initiation in vivo on Xenopus laevis ribosomal DNAProceedings of the National Academy of Sciences, 1977
- The Ribosomal RNA CistronsProgress in Nucleic Acid Research and Molecular Biology, 1971
- Extrachromosomal nucleolar genes in amphibian oocytes.1969