Monensin and forskolin inhibit the transcription rate of sucrase‐isomaltase but not the stability of its mRNA in Caco‐2 cells
- 9 August 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 328 (1-2) , 55-58
- https://doi.org/10.1016/0014-5793(93)80964-v
Abstract
Treatment of Caco‐2 cells with forskolin (25 μM) or monensin (1 μM) has previously been shown to cause a marked decrease in the level of sucrase‐isomaltase (SI) mRNA, without any effect on the expression of dipeptidylpeptidase IV (DPP‐IV). In the present work, we report that there is no significant difference in the stability of SI mRNA between control and treated cells. On the other hand, we demonstrate a decrease in the transcription rate of SI mRNA which is sufficient to account for the decrease in the steady‐state level of SI mRNA both in forskolin‐ and monensin‐treated Caco‐2 cells.Keywords
This publication has 21 references indexed in Scilit:
- Decrease of mRNA levels and biosynthesis of sucrase-isomaltase but not dipeptidylpeptidase IV in forskolin or monensin-treated Caco-2 cellsCellular and Molecular Life Sciences, 1991
- Isolation of a cDNA probe for the human intestinal dipeptidylpeptidase IV and assignment of the gene locus DPP4 to chromosome 2Annals of Human Genetics, 1990
- Reversible forskolin‐induced impairment of sucrase‐isomaltase mRNA levels, biosynthesis, and transport to the brush border membrane in Caco‐2 cellsJournal of Cellular Physiology, 1989
- Poly(A), poly(A) binding protein and the regulation of mRNA stabilityTrends in Biochemical Sciences, 1989
- Transcriptional Regulation in Mammalian Cells by Sequence-Specific DNA Binding ProteinsScience, 1989
- Monensin inhibits the expression of sucrase‐isomaltase in Caco‐2 cells at the mRNA levelFEBS Letters, 1988
- On/off regulation of gene expression at the level of splicingTrends in Genetics, 1988
- Inhibition of the post‐translational processing of microvillar hydrolases is associated with a specific decreased expression of sucrase‐isomaltase and an increased turnover of glucose in Caco‐2 cells treated with monensinFEBS Letters, 1986
- Enterocytic differentiation and glucose utilization in the human colon tumor cell line Caco‐2: Modulation by forskolinJournal of Cellular Physiology, 1985
- Perturbation of vesicular traffic with the carboxylic ionophore monensinCell, 1983