Distribution and Excretion of Radioactivity in Rats After Oral Administration of H3-3-Methylcholanthrene.

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Abstract
Sprague-Dawley female rats, age 50 days, were used in 2 separate experiments. In the first experiment, selected tissues, urine and feces were analyzed for radioactivity 24 hrs. after the oral administration of 10 mg H3-3-methylcholanthrene (2,000,000 disintegrations/min./mg) in 1 ml sesame oil. Results obtained by the combustion technique of Jacobson et al. (1960) for determination of total radioactivity and those obtained by toluene extraction indicate that fat and mammary gland contained mainly toluene-extractable radioactive material, whereas negligible quantities of the total radio-activity in liver, kidney, lung, brain, uterus and skeletal muscle were toluene-extractable. These results suggest that the administered hydrocarbon is rapidly converted to protein-bound metabolites. In the 2d experiment, 24-hr. samples of urine and feces were analyzed for total radioactivity following oral administration of a single 10 mg dose of H3-3-methylcholanthrene. Of the total radioactivity 64% was recovered in feces and 8% in urine during the first 7 days of observation. Trace amounts of radioactivity were found in urine and feces during the remainder of the observation period (18 days).