Digital composition of images with increased depth of focus considering depth information

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Abstract
Two digital image processing methods, capable of composing microscope images with increased depth of focus and of mapping 3-D distribution on the altitude or depth of the thick object, are presented: one uses pseudocolor display of focal-series images, and the other considers the local variance of each of the focal-series images. Experimental results demonstrate the usefulness of both methods. The high-speed digital image processing system developed by the authors plays an important role in both methods.