Liver Sinusoidal Endothelial Cells Are Not Permissive for Adenovirus Type 5
- 10 February 2000
- journal article
- research article
- Published by Mary Ann Liebert Inc in Human Gene Therapy
- Vol. 11 (3) , 481-486
- https://doi.org/10.1089/10430340050015941
Abstract
Adenoviral vectors are known to transduce hepatocytes in normal liver tissue with high efficiency. The aim of this study was to investigate whether sinusoidal endothelial cells, which separate hepatocytes from the bloodstream in the sinusoidal lumen, are permissive for infection by adenoviruses. We show here that microvascular liver sinusoidal endothelial cells are not infected by adenovirus type 5 in vivo or in vitro unless high MOIs are used. In contrast, macrovascular endothelial cells from aorta are efficiently infected by adenovirus type 5. In addition, Kupffer cells, similar to sinusoidal endothelial cells, are not infected by adenovirus type 5. Liver sinusoidal endothelial cells do not express the integrin receptor alpha vbeta3, which is required for efficient infection by adenoviruses. Our results demonstrate that hepatocytes are the main cell population of the liver that is infected by adenovirus type 5.Keywords
This publication has 26 references indexed in Scilit:
- Induction of cytokine production in naive CD4+ T cells by antigen-presenting murine liver sinusoidal endothelial cells but failure to induce differentiation toward Th1 cells☆, ☆☆Gastroenterology, 1999
- Adenovirus-Mediated Gene Transfer to Lower Limb Artery of Patients with Chronic Critical Leg IschemiaHuman Gene Therapy, 1998
- Enhanced in vivo adenovirus-mediated gene transfer to rat hepatocarcinomas by selective administration into the hepatic arteryGene Therapy, 1998
- Adenovirus-mediated wild-type p53 overexpression inhibits endothelial cell differentiation in vitro and angiogenesis in vivoGene Therapy, 1998
- Prevention of bleomycin-induced pulmonary fibrosis after adenovirus-mediated transfer of the bacterial bleomycin resistance gene.Journal of Clinical Investigation, 1997
- Adenoviral Delivery of Recombinant DNA into Transgenic Mice Bearing Hepatocellular CarcinomasHuman Gene Therapy, 1996
- Human Kupffer cells secrete IL-10 in response to lipopolysaccharide (LPS) challengeJournal of Hepatology, 1995
- Prostanoid release of cultured liver sinusoidal endothelial cells in response to endotoxin and tumor necrosis factorJournal of Hepatology, 1990
- The Liver Sieve: Considerations Concerning the Structure and Function of Endothelial Fenestrae, the Sinusoidal Wall and the Space of DisseHepatology, 1985
- Separation of Kupffer and endothelial cells of the rat liver by centrifugal elutriationExperimental Cell Research, 1976