Expression of ?-d-mannosidase in man-hamster somatic cell hybrids
- 1 June 1977
- journal article
- research article
- Published by Springer Nature in Biochemical Genetics
- Vol. 15 (5-6) , 455-476
- https://doi.org/10.1007/bf00520191
Abstract
Two types of α-d-mannosidase isozymes are present in human white blood cells, human diploid fibroblasts, and HeLa cells. One of these (the S isozyme) constitutes the major α-d-mannosidase of the human cells, has a pH optimum of 4.4, and is associated with lysosomes. The other (the F isozyme) is most active at pH 6, is acid labile, and is located in the soluble portion of the cytoplasm. The expression of human lysosomal α-d-mannosidase was examined in man-hamster hybrid clones, and was found to be concordant with that of phosphohexose isomerase in 54 of 55 primary clones. A locus specifying human lysosomal α-d-mannosidase has therefore been assigned to chromosome 19.Keywords
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