The Effect of Tube-Wall Finish on HPLC Column Performance
- 1 August 1984
- journal article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 22 (8) , 332-334
- https://doi.org/10.1093/chromsci/22.8.332
Abstract
HPLC column performance is shown to be unaffected by the finish of the column tube. This finding is in direct contradiction to the commonly held belief that using a polished column tube will enhance performance by minimizing the wall effect. Stainless-steel column tubes with five different finishes of 4- to 10-, 16-, 25-, 32-, and 45- to 60-microinch wall were packed with 5-μ spherical, reversed-phase media. Efficiency and peak-symmetry values were statistically identical for all columns.Keywords
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