Studies on Erythrocyte Glycolysis

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Abstract
The rate of increase or decrease of 2, 3-diphosphoglycerate in red cells was measured under various conditions. When the cells were incubated with carbon monoxide, the diphosphoglycerate concentration decreased with a rate of 0.9μmoles/ml cells per hr. In the presence of sodium fluoride when the glycolysis stopped completely, the concentration remains constant. The transition from anaerobic to aerobic state did not change the concentration, when preincubated with fluoride. When incubated with bisulfite, the concentration decreased with a rate of 1.6μmole/ml cells per hr and disappeared completely in 4 hr. With arsenate, the concentration decreased with a rate of 0.7μmoles/ml cells per hr and with inosine and pyruvate at elevated pH, the concentration increased with a rate of 0.8μmoles/ml cells per hr and reached a value of 17 mM. These rates of increase or decrease of the diphosphoglycerate concentration correspond to 25 to 50 percent of the glycolytic flow, which may indicate the maximal estimate of the diphosphoglycerate by-pass. Factors determining the diphosphoglycerate concentration in red blood cells were discussed.

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