RNA/DNA hybrid duplexes with identical nearest‐neighbor base‐pairs have identical stability
- 18 October 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 354 (1) , 74-78
- https://doi.org/10.1016/0014-5793(94)01098-6
Abstract
Energetic behaviors of eight pairs of RNA/DNA hybrid duplexes with identical nearest neighbors have been investigated by UV melting analysis. In the pairs with identical nearest-neighbor pairs, the melting curve traces at the same strand concentration were very similar. The average difference in stabilization energy of these pairs was 4%, which was about expected within experimental error. These results indicate that the nearest-neighbor model is valid for predicting the stability of RNA/DNA hybrid duplexes as well as RNA/RNA and DNA/DNA duplexesKeywords
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