HETEROGENEITY OF HLA-A,B, IA-LIKE, AND MELANOMA-ASSOCIATED ANTIGEN EXPRESSION BY HUMAN-MELANOMA CELL-LINES ANALYZED WITH MONOCLONAL-ANTIBODIES AND FLOW-CYTOMETRY

  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (10) , 4110-4115
Abstract
With the use of monoclonal antibodies, indirect immunofluorescence and flow cytometry, human melanoma cell lines (Colo-38, M-16, and M-21) were examined for the quantitative expression of HLA-A,B antigens, Ia-like antigens and 2 human melanoma-associated antigens (MAA) referred to as 280K [kilodalton] and 94K MAA. Each of the melanoma cell lines examined showed tremendous heterogeneity with regard to antigen expression. A detailed study of the heterogeneity of the 4 antigens listed revealed that variation in cell size could in part account for the large differences in antigen expression. Cell surface density of HLA-A,B antigens, Ia-like antigens and the 280K and 94K MAA remained relatively constant over a wide range of cell sizes that were examined, with the exception that small melanoma cells showed a slightly lower mean surface density of MAA expression than did large cells. A novel method was used to detect the expression of the 2 MAA as a function of the Colo-38 cell cycle using dual-laser beam flow cytometry. The 280K MAA and the 94K MAA are differentially expressed during various stages of the cell cycle, with each antigen being maximally detected during G2 + M.

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