BONE MARROW TRANSPLANTATION AFTER LETHAL CHEMOTHERAPY IN “NUDE” MICE1

Abstract
Isogeneic bone marrow transplantation was performed in nude and normal C3H mice after lethal doses of dimethylmyleran (DMM), meso-DMM and (.+-.)-DMM were given. Nude mice were not more sensitive to DMM or its isomers than normal mice. With adequate cell doses in the range of 3 .times. 106 to 30 .times. 106 bone marrow cells or 10 .times. 106 to 30 .times. 106 spleen cells, the survival rate after lethal chemotherapy was not significantly different between nude mice reconstituted with a graft from nude donors and normal mice reconstituted with a graft from normal donors. Only with suboptimal cell doses (1 .times. 106 bone marrow cells) was survival superior in normal mice. The hemopoietic capacity was not conferred to the nude donor cells by an extrinsic factor. Nude mice accepted bone marrow grafts from allogeneic nude donors.