Detection and quantification of IgM, IgA, IgG1and IgG2antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay
- 1 February 1981
- journal article
- research article
- Published by Cambridge University Press (CUP) in Epidemiology and Infection
- Vol. 86 (1) , 79-85
- https://doi.org/10.1017/s0022172400068765
Abstract
SUMMARY: A simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (FMD) virus in bovine sera. The assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. The specific antibody is reacted with FMD virus, which is then detected by an enzyme-labelled anti virus IgG.Keywords
This publication has 7 references indexed in Scilit:
- Enzyme-labelled immunosorbent assay techniques in foot-and-mouth disease virus researchEpidemiology and Infection, 1978
- EVALUATION OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR QUANTITATION BY SUBCLASS FOR BOVINE ANTIBODIES1978
- Radial immuno-diffusion and serum-neutralisation techniques for the assay of antibodies to swine vesicular diseaseResearch in Veterinary Science, 1976
- Coupling of enzymes to proteins with glutaraldehydeImmunochemistry, 1969
- Further Studies of the Early Antibody in the Sera of Cattle and Guinea Pigs Infected with Foot-and-Mouth Disease VirusThe Journal of Immunology, 1964
- Purification of Radioactive Foot-and-Mouth Disease VirusNature, 1963