Human Monoclonal Antibodies Neutralizing Varicella-Zoster Virus
- 1 August 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Infectious Diseases
- Vol. 152 (2) , 280-285
- https://doi.org/10.1093/infdis/152.2.280
Abstract
Hybridomas secreting human monoclonal antibodies to varicella-zoster virus (VZV) were produced by fusing B cells of a patient recovering from acute varicella infection with a human-mouse cell line. Two hybrid lines have continued to secrete IgG1, one with .kappa. and the other with .lambda. chains for at least 12 mo. Each antibody neutralizes virus infectivity between 1-5 .mu.g of partially purified Ig/ml, each shows a different pattern of immunofluorescent staining of virus-infected cells, and 1 identifies 3 viral proteins with MW of 60,000, 95,000 and 97,000.This publication has 3 references indexed in Scilit:
- Rescue of human monoclonal antibody production from an EBV-transformed B cell line by fusion to a human-mouse hybridomaJournal of Immunological Methods, 1984
- Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.Proceedings of the National Academy of Sciences, 1980
- Immunoglobulins M and G to varicella-zoster virus measured by solid-phase radioimmunoassay: antibody responses to varicella and herpes zoster infectionsJournal of Clinical Microbiology, 1980