An Improved Method for Perforated Patch Recordings Using Nystatin-Fluorescein Mixture.
Open Access
- 1 January 1993
- journal article
- Published by Physiological Society of Japan in The Japanese Journal of Physiology
- Vol. 43 (2) , 267-273
- https://doi.org/10.2170/jjphysiol.43.267
Abstract
This paper describes an improved method for solubilizing nystatin in an aqueous solution without the aid of organic solvents. The patch pipette was filled with a solution of nystatin and fluorescein sodium mixed in a molar ratio of 1:10. The success rate of perforated patch recordings was substantially improved, and the access resistance of 20-40 M omega was readily achieved.Keywords
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