Nonisotopic, Semiautomated Plasmodium falciparum Bioassay for Measurement of Antimalarial Drug Levels in Serum or Plasma
- 1 November 2004
- journal article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 48 (11) , 4485-7
- https://doi.org/10.1128/aac.48.11.4485-4487.2004
Abstract
A simple, nonisotopic, semiautomated bioassay for the measurement of antimalarial drug levels in plasma or serum based on the quantitation of histidine-rich protein II in malaria culture is presented. The assay requires only small sample volumes and was found to be highly sensitive and reproducible. The results closely paralleled those obtained with isotopic bioassays ( R = 0.988, P < 0.001) and high-performance liquid chromatography-electrochemical detection ( R = 0.978, P < 0.001).Keywords
This publication has 10 references indexed in Scilit:
- Plasmodium falciparum-Based Bioassay for Measurement of Artemisinin Derivatives in Plasma or SerumAntimicrobial Agents and Chemotherapy, 2004
- Comparison of bioassay and high performance liquid chromatographic assay of artesunate and dihydroartemisinin in plasmaActa Tropica, 2003
- Malaria drug-sensitivity testing: new assays, new perspectivesTrends in Parasitology, 2003
- Histidine-Rich Protein II: a Novel Approach to Malaria Drug Sensitivity TestingAntimicrobial Agents and Chemotherapy, 2002
- Comparative pharmacokinetics and effect kinetics of orally administered artesunate in healthy volunteers and patients with uncomplicated falciparum malaria.The American Journal of Tropical Medicine and Hygiene, 2001
- A bioassay for evaluating antimalarial activity and for measuring concentration in plasma.Tropical Medicine & International Health, 1997
- Clinical pharmacokinetics and pharmacodynamics of artemisinin and derivativesTransactions of the Royal Society of Tropical Medicine and Hygiene, 1994
- Chloroquine Bioassay using Malaria MicroculturesThe American Journal of Tropical Medicine and Hygiene, 1993
- A bioassay for derivatives of qinghaosu (artemisinin).1992
- Determination of the Antimalarial Arteether and its Deethylated Metabolite Dihydroartemisinin in Plasma by High-Performance Liquid Chromatography with Reductive Electrochemical DetectionJournal of Pharmaceutical Sciences, 1991