Transcriptional control of herpesvirus gene expression: gene functions required for positive and negative regulation.
Open Access
- 1 January 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (2) , 256-260
- https://doi.org/10.1073/pnas.83.2.256
Abstract
We have used an in vitro nuclear run-off assay to measure the levels of transcription of specific herpes simplex virus genes at different times during a lytic infection. We analyzed the effects of inhibition of DNA replication and of defects in two herpes simplex virus regulatory proteins on the transcription of these genes. We present evidence that the transcription of the .alpha.ICP4 gene is negatively regulated during a lytic infection. The regulation of ICP4 gene transcription requires the .beta. protein ICP8 (where ICP = infected cell polypeptide). Transcription of the .beta.ICP8, .gamma.1ICP5, and .gamma.2 glycoprotein C (gC) genes was dependent on ICP4, and transcription of the .gamma.2gC gene was strongly inhibited when DNA replication was blocked. Defects in ICP8 also resulted in increased levels of transcription of the ICP4, ICP8, ICP5, and gC genes from parental viral genomes. Our results suggest that ICP8 may be important in maintaining the highly ordered cascade of viral gene expression.This publication has 57 references indexed in Scilit:
- Viral DNA synthesis is required for the efficient expression of specific herpes simplex virus type 1 mRNA speciesPublished by Elsevier ,2004
- Identification of herpes simplex virus DNA sequences which encode a trans-acting polypeptide responsible for stimulation of immediate early transcriptionJournal of Molecular Biology, 1984
- Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogeneNature, 1984
- Regulation of α genes of herpes simplex virus: Expression of chimeric genes produced by fusion of thymidine kinase with α gene promotersCell, 1981
- A herpes simplex virus type 1 function continuously required for early and late virus RNA synthesisNature, 1980
- Molecular genetics of herpes simplex virus: Demonstration of regions of obligatory and nonobligatory identity within diploid regions of the genome by sequence replacement and insertionProceedings of the National Academy of Sciences, 1978
- Temporal regulation of herpes simplex virus type 1 transcription: location of transcripts on the viral genomePublished by Elsevier ,1977
- Mode of inhibition of Herpes simplex virus DNA polymerase by phosphonoacetateBiochemistry, 1975
- Regulation of herpesvirus macromolecular synthesis: sequential transition of polypeptide synthesis requires functional viral polypeptides.Proceedings of the National Academy of Sciences, 1975
- Regulation of Herpesvirus Macromolecular Synthesis: Nuclear Retention of Nontranslated Viral RNA SequencesProceedings of the National Academy of Sciences, 1974