Using Secretion to Solve a Solubility Problem: High-Yield Expression in Escherichia coli and Purification of the Bacterial Glycoamidase PNGase F
- 1 February 2002
- journal article
- Published by Elsevier in Protein Expression and Purification
- Vol. 24 (1) , 90-98
- https://doi.org/10.1006/prep.2001.1555
Abstract
No abstract availableKeywords
This publication has 14 references indexed in Scilit:
- Active Site and Oligosaccharide Recognition Residues of Peptide-N4-(N-acetyl-β -D-glucosaminyl)asparagine Amidase FPublished by Elsevier ,1995
- The three-dimensional structure of PNGase F, a glycosyl asparaginase from Flavobacterium meningosepticumStructure, 1994
- Crystal Structure of Peptide-N4-(N-acetyl-.beta.-D-glucosaminyl)asparagine amidase F at 2.2-.ANG. ResolutionBiochemistry, 1994
- Purification and Crystallization of the Endoglycosidase PNGase F, a Peptide:N-glycosidase from Flavobacterium meningosepticumJournal of Molecular Biology, 1994
- Peptide‐N4‐(N‐acetyl‐β‐glucosaminyl)asparagine amidase F cannot release glycans with fucose attached α1 → 3 to the asparagine‐linked N‐acetylglucosamine residueEuropean Journal of Biochemistry, 1991
- [6] Use of T7 RNA polymerase to direct expression of cloned genesPublished by Elsevier ,1990
- [31] Expression and secretion of foreign proteins in Escherichia coliPublished by Elsevier ,1987
- A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.Proceedings of the National Academy of Sciences, 1985
- endo-beta-N-acetylglucosaminidase F: endoglycosidase from Flavobacterium meningosepticum that cleaves both high-mannose and complex glycoproteins.Proceedings of the National Academy of Sciences, 1982
- An efficient and reproducible procedure for the formation of spheroplasts from variously grown Escherichia coliAnalytical Biochemistry, 1976