Preparation and Orientation of Canine Retinal Vasculature A Modified Trypsin Digestion Technique

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Abstract
An existing technique was modified in order to demonstrate the oriented vasculature of the entire canine retina, and described for use by persons unfamiliar with this exacting technique. The or a ciliaris retinae of the canine retina is irregular in outline and the insertion of the optic nerve, is eccentric. When the eye is sectioned into quadrants, their retinal radii and included areas are of differing dimensions. Prominent retinal veins are visible through the dissecting microscope at various stages of the dissection and trypsin digestion procedure. These distinctive vascular patterns and varying radial lengths are recorded diagrammatically to permit the identification of the vitreal aspect of each retinal quadrant. The retinal vasculature is presented in its correct orientation in completed slide preparations. Orientation of retinal vasculature permits correlation with previous clinical and photographic studies, and enhances an evaluation of normal and abnormal histology and cytological parameters.

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