False-Positive Rabbit Ileal Loop Reactions Attributed to Vibrio parahaemolyticus Broth Filtrates

Abstract
Vibrio parahaemolyticus broth filtrates have previously been shown to produce positive reactions in rabbit ileal loops only if concentrated 10-fold by lyophilization. This method of concentration produces solutions that contain V. parahaemolyticus, a finding which suggests that previous studies require further evaluation. Since most culture media contain 0.5% NaCl, it is important to determine and to control the NaCl content and the osmolality of all lyophilized concentrates tested in the ligated rabbit ileum.

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