Transketolase Activity in Tissues of Uremic Rats

Publisher Website
Google Scholar
Abstract
The activity of the enzyme transketolase (TK) was measured in rats made uremic by surgical destruction of renal tissue. These uremic animals together with sham-operated control rats were pair-fed diets containing 6%, 18% or 23% protein. Pair-fed rats were sacrificed when the urea clearance of the uremic rat was below 0.5 ml/minute. TK was measured in red cells, liver and brain. The inhibitory effect of uremic plasma on TK was determined by incubating red cell hemolysates from controls with plasma from control and uremic rats. TK activity of erythrocytes was higher in uremic than in control rats. TK from either liver or brain did not differ in control and uremic animals. No inhibitory effect of plasma from uremic rats on TK or erythrocytes was observed.