Characterization of the C-type Virus Produced In Vitro by MOPC-21 BALB/c Myeloma

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Abstract
The tissue culture-adapted P3 clone of MOPC-21 BALB/c [mouse] myeloma cells produce a C-type virus, designated as MO21-MuMAV, that has many properties in common with the NB-tropic, C-type virus produced by a clone of the FLOPC-1 line of BALB/c myeloma (FL1-MuMAV) and 1 property that is distinctly different. Both particles are extremely unstable as shown by their buoyant densities when analyzed under different conditions of isopycnic gradient centrifugation; the enzymic activity of the virus RNA-dependent DNA polymerase is similar; the 2 viruses are similar antigenically; both viruses have similar [rat sarcoma] XC [cell] syncytium-inducing activity; the endogenous RNA of both viruses contains poly(A) tracts of a similar length; both are NB-tropic as they productively infected [mouse fibroblast] BALB/3T3 and NIH/3T3 cells. The RNA of MO21-MuMAV consists of more high MW (60-70S) RNA species than does FL1-MuMAV RNA. MO21-MuMAV does not infect normal rat kidney cells as efficiently as does FL1-MuMAV. The NB-tropism of MO21-MuMAV does not appear to be due to a mixture of N- and B-tropic viruses on the basis of the NB-tropism of the progeny virus produced by infected NIH/3T3 and BALB/3T3 cells, and the dose-response relationship of MO21-MuMAV infection of BALB/3T3 and NIH/3T3 cells. Thus, different BALB/c myelomas probably produce C-type viruses that are very similar, but not necessarily identical.

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