Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation
- 1 December 1981
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 6 (4) , 283-292
- https://doi.org/10.1007/bf02798279
Abstract
Magnetic biospecific affinity adsorbents for immunoglobulin and enzyme isolation have been prepared. They were obtained by a “ post-magnetization” procedure involving a simple treatment of the various affinity gels with magnetic ferrofluid. The magnetic biospecific adsorbents tested include magnetic protein A-Sepharose for isolation of IgG antibodies, magnetic human serum albumin (HSA)-Sepharose for anti-HSA isolation, and magnetic 2′,5′-ADP for isolation of glucose-6-phosphate dehydrogenase from baker’s yeast and hemolyzates of human red blood cells. For the latter enzyme, a 11,000-fold purification was achieved in one step.Keywords
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