Coupling site-directed mutagenesis with high-level expression: large scale production of mutant porins fromE. coli
Open Access
- 1 June 1998
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 163 (1) , 65-72
- https://doi.org/10.1111/j.1574-6968.1998.tb13027.x
Abstract
Combination of an origin repair mutagenesis system with a new mutS host strain increased the efficiency of mutagenesis from 46% to 75% mutant clones.Keywords
This publication has 24 references indexed in Scilit:
- Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genesPublished by Elsevier ,2004
- Voltage sensing in the PhoE and OmpF outer membrane porins of Escherichia coli: role of charged residuesJournal of Molecular Biology, 1997
- Replacement of the Sole Histidinyl Residue in OmpF Porin fromE. coliby Threonine (H21T) Does Not Affect Channel Structure and FunctionBiochemical and Biophysical Research Communications, 1996
- Crystallization of Monodisperse Maltoporin from Wild-Type and Mutant Strains of Various EnterobacteriaceaeBiochemical and Biophysical Research Communications, 1994
- Transport Proteins in Bacteria: Common Themes in Their DesignScience, 1992
- Mutagenesis by random linker insertion into the lamB gene of Escherichia coli K12Molecular Genetics and Genomics, 1986
- Periplasmic accumulation of truncated forms of outer-membrane PhoE protein of Escherichia coli K-12Journal of Molecular Biology, 1986
- Isolation and characterization of mutants deleted for thesulA-ompAregion of theEscherichia coliK-12 chromosomeFEMS Microbiology Letters, 1986
- OmpF porin synthesis inEscherichia colistrains B and K-12 carrying heterologousompBand/orompFlociFEMS Microbiology Letters, 1983
- Electrophoretic resolution of the ‘major outer membrane protein’ of Escherichia coli K12 into four bandsFEBS Letters, 1975