Electrophoresis in a density gradient

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Abstract
A density-gradient electrophoresis apparatus somewhat modified from that designed by Svensson is described. Experiments were carried out in gradients made frog ethanol and from sucrose. The reproducibility of movement and shape of bands of human albumin and the completeness of separation of this protein from a band of hemoglobin were studied in the sucrose gradient. The specific activities of the fractions thus obtained from human plasma albumin trace-iodinated with I131 varied considerably and showed a maximum just preceding the maximum protein concentration as measured by UV- light absorption. Insulin was caused to migrate in both the sucrose and the ethanol gradients; in the latter the protein concentration curve possessed a shoulder. Suitable conditions were found for the electrophoresis of slow-reacting substance A, a substance causing constriction of the guinea pig ileum.