Further Studies on the Gastric Secretory Excitant from the Pyloric Mucosa.

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Abstract
Summary.: A gastric secretory excitant is isolated from the pyloric mu‐cosa of cat and pig. The agent is of protein nature.Two reliable methods for the preparation of the active principle in a relatively pure state are described. The methods involve the precipitation of HCl‐mucosall extracts with NaCl and further precipitation with tannic acid or trichloracetic acid. Inert material is removed by washing with 80 % acid alcohol and iso‐electrical precipitation at pH 8.0. Finally the active material is precipitated with trichloracetic acid.The active agent selectively activates the gastric glands, the gastric juice obtained being strongly acid but poor in pepsin. Salivary, pancreatic or bile secretion are not influenced by the agent.The gastric secretagogue shows physiological as well as chemical similarities to secretin. Identity with the supposed gastric hormone, gastrin, is probable.