Phosphatidic Acid and Glyceride Synthesis by Particles from Spinach Leaves

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Abstract
The enzymic synthesis of phosphatidic acid, tri-, di-, and monoglyceride from L-[alpha]-glycerol-phosphate-l,3-C14 is described. On a specific acitvity basis a fraction from spinach leaves sedimenting at 104,000 x g had the greatest activity. Requirements for ATP, coenzyme A, fatty acid, and a divalent cation were demonstrated, thus suggesting synthesis of phosphatidic acid by the Kennedy-Kornberg pathway. Replacement of glycerol-phosphate with glycerol resulted in a pronounced decrease of incorporation. Activity could be restored by addition of glycerol kinase thus indicating absence of glycerol kinase in these particles. Kinetic studies on the products indicated that phosphatidic acid was the precursor for glycerides. In addition to these products an unknown labeled phosphatide is observed.