Catalysis of the peptide bond formation by 50 S subunits of E. Coli ribosomes with N-(formyl) methionine ester of adenylic acid as peptide donor
- 1 November 1976
- journal article
- research article
- Published by Springer Nature in Molecular Biology Reports
- Vol. 3 (2) , 151-156
- https://doi.org/10.1007/bf00423229
Abstract
50 S subunits of E. coli ribosomes catalyze the reaction of the 2′(3′)-N-(formyl) methionine ester of adenosine 5′-phosphate and Phe-tRNA resulting in peptide bond synthesis. Cytidine 5′-phosphate stimulates this process on 50 S ribosomal subunits as well as on intact ribosomes. The obtained data show that the areas of the peptidyltransferase donor site which binds the 3′-terminal fragment of peptidyl-tRNA possess completely formed structures on 50 S ribosomal subunits.Keywords
This publication has 12 references indexed in Scilit:
- The study of the stimulating mechanism of the peptidyl donor activity of 2′(3′)‐O‐(N‐formylmethionyl)‐adenosine‐5′‐phosphate in peptidyl transferase of E. coli ribosomeFEBS Letters, 1976
- Aminoacyl derivatives of nucleosides, nucleotides, and polynucleotides. XXII. Substrate specificity of ribosomal peptidyltransferase. Effect of modification in the heterocyclic, carbohydrate and amino acid moiety of 2'(3')-O-L-phenylalanyladenosineBiochemistry, 1975
- Effect of cytidine‐5′‐monophosphate on peptidyl transferase activityFEBS Letters, 1975
- Peptidyl transferase centre of bacterial ribosomes: substrate specificity and binding sitesNucleic Acids Research, 1975
- Characterization of cytosol phosphopeptidesFEBS Letters, 1973
- Interaction of transfer RNA with ribosomes in the absence of messengerBiochemical and Biophysical Research Communications, 1972
- Reaction of puromycin with N-acetylphenylalanyl-tRNA on ribosomes reassociated from escherichia coli ribosomal subunitsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Ribosome-catalysed peptidyl transfer: the polyphenylalanine systemJournal of Molecular Biology, 1968
- Catalysis of peptide bond formation by 50 s ribosomal subunits from Escherichia coliJournal of Molecular Biology, 1967
- The puromycin reaction and its relation to protein synthesisJournal of Molecular Biology, 1964