A miniaturized counting technique for anaerobic bacteria
- 1 December 1976
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 22 (12) , 1728-1733
- https://doi.org/10.1139/m76-255
Abstract
A miniaturized counting technique gave results as good as the pour-plate and Most Probable Number (MPN) techniques for enumeration of Clostridia spp. and anaerobic isolates from the gut. Highest counts were obtained when ascorbic acid (1%) and dithiothreitol (0.015%) were added to the reinforced clostridial medium used for counting. This minimized the effect of exposure to air before incubation. The miniature technique allowed up to 40 samples to be plated and incubated in one McIntosh-Filde's-type anaerobic jar, compared with 3 or 4 by the normal pour plate.This publication has 1 reference indexed in Scilit:
- Dithiothreitol, a New Protective Reagent for SH Groups*Biochemistry, 1964